Construct: shRNA TRCN0000381663

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_002346410.1-218s21c1
DNA Barcode:
CCAAACCTCTCTGGCATGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ROCK1P1 (727758)
Gene Description:
Rho associated coiled-coil containing protein kinase 1 pseudogene 1
Transcript:
RefSeq XM_002346410.1 (NON-CURRENT)
Match location:
Position 218 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000381663)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAACCTCTCTGGCATGTTT
Hairpin Sequence:
5'-CCGG-CCAAACCTCTCTGGCATGTTT-CTCGAG-AAACATGCCAGAGAGGTTTGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAACCTCTCTGGCATGTTTCTCGAGAAACATGCCAGAGAGGTTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAACCTCTCTGGCATGTTTCTCGAGAAACATGCCAGAGAGGTTTGG-3'

Other clones with same target sequence:

(none)