Construct: shRNA TRCN0000381719

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001080826.1-2348s21c1
DNA Barcode:
ACAGCGGGAAGAAGCTCTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRAG1 (157285)
Gene Description:
PEAK1 related, kinase-activating pseudokinase 1
Transcript:
RefSeq NM_001080826.1 (NON-CURRENT)
Match location:
Position 2348 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000381719)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAGCGGGAAGAAGCTCTTTG
Hairpin Sequence:
5'-CCGG-ACAGCGGGAAGAAGCTCTTTG-CTCGAG-CAAAGAGCTTCTTCCCGCTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAGCGGGAAGAAGCTCTTTGCTCGAGCAAAGAGCTTCTTCCCGCTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAGCGGGAAGAAGCTCTTTGCTCGAGCAAAGAGCTTCTTCCCGCTGT-3'

Other clones with same target sequence:

(none)