Construct: shRNA TRCN0000381834

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138453.2-159s21c1
DNA Barcode:
ACTCCTCTGATCAGAACTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RAB3C (115827)
Gene Description:
RAB3C, member RAS oncogene family
Transcript:
RefSeq NM_138453.2 (NON-CURRENT)
Match location:
Position 159 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000381834)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTCCTCTGATCAGAACTTTG
Hairpin Sequence:
5'-CCGG-ACTCCTCTGATCAGAACTTTG-CTCGAG-CAAAGTTCTGATCAGAGGAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTCCTCTGATCAGAACTTTGCTCGAGCAAAGTTCTGATCAGAGGAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTCCTCTGATCAGAACTTTGCTCGAGCAAAGTTCTGATCAGAGGAGT-3'

Other clones with same target sequence:

(none)