Construct: shRNA TRCN0000381837

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003762.3-332s21c1
DNA Barcode:
GGACCATCTGGACCAAGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VAMP4 (8674)
Gene Description:
vesicle associated membrane protein 4
Transcript:
RefSeq NM_003762.3 (NON-CURRENT)
Match location:
Position 332 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000381837)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGACCATCTGGACCAAGATTT
Hairpin Sequence:
5'-CCGG-GGACCATCTGGACCAAGATTT-CTCGAG-AAATCTTGGTCCAGATGGTCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGACCATCTGGACCAAGATTTCTCGAGAAATCTTGGTCCAGATGGTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGACCATCTGGACCAAGATTTCTCGAGAAATCTTGGTCCAGATGGTCC-3'

Other clones with same target sequence:

  1. TRCN0000348352