Construct: shRNA TRCN0000381899

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172120.3-2123s21c1
DNA Barcode:
CATCGACAAACCACCATTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vps41 (218035)
Gene Description:
VPS41 HOPS complex subunit
Transcript:
RefSeq NM_172120.3 (NON-CURRENT)
Match location:
Position 2123 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000381899)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCGACAAACCACCATTTAT
Hairpin Sequence:
5'-CCGG-CATCGACAAACCACCATTTAT-CTCGAG-ATAAATGGTGGTTTGTCGATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCGACAAACCACCATTTATCTCGAGATAAATGGTGGTTTGTCGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCGACAAACCACCATTTATCTCGAGATAAATGGTGGTTTGTCGATG-3'

Other clones with same target sequence:

(none)