Construct: shRNA TRCN0000381901

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004371.3-786s21c1
DNA Barcode:
TAACAGGGCACAACCATTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COPA (1314)
Gene Description:
COPI coat complex subunit alpha
Transcript:
RefSeq NM_004371.3 (NON-CURRENT)
Match location:
Position 786 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000381901)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAACAGGGCACAACCATTATG
Hairpin Sequence:
5'-CCGG-TAACAGGGCACAACCATTATG-CTCGAG-CATAATGGTTGTGCCCTGTTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAACAGGGCACAACCATTATGCTCGAGCATAATGGTTGTGCCCTGTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAACAGGGCACAACCATTATGCTCGAGCATAATGGTTGTGCCCTGTTA-3'

Other clones with same target sequence:

(none)