Construct: shRNA TRCN0000381997

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020185.3-858s21c1
DNA Barcode:
AGCATGAGGTCCATCAGTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DUSP22 (56940)
Gene Description:
dual specificity phosphatase 22
Transcript:
RefSeq NM_020185.3 (NON-CURRENT)
Match location:
Position 858 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000381997)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCATGAGGTCCATCAGTATC
Hairpin Sequence:
5'-CCGG-AGCATGAGGTCCATCAGTATC-CTCGAG-GATACTGATGGACCTCATGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCATGAGGTCCATCAGTATCCTCGAGGATACTGATGGACCTCATGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCATGAGGTCCATCAGTATCCTCGAGGATACTGATGGACCTCATGCT-3'

Other clones with same target sequence:

(none)