Construct: shRNA TRCN0000382048

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080737.2-466s21c1
DNA Barcode:
GGCGACTAAAGAATGAGTTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYTL4 (94121)
Gene Description:
synaptotagmin like 4
Transcript:
RefSeq NM_080737.2 (NON-CURRENT)
Match location:
Position 466 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000382048)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCGACTAAAGAATGAGTTAC
Hairpin Sequence:
5'-CCGG-GGCGACTAAAGAATGAGTTAC-CTCGAG-GTAACTCATTCTTTAGTCGCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCGACTAAAGAATGAGTTACCTCGAGGTAACTCATTCTTTAGTCGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCGACTAAAGAATGAGTTACCTCGAGGTAACTCATTCTTTAGTCGCC-3'

Other clones with same target sequence:

(none)