Construct: shRNA TRCN0000382113

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007347.3-2251s21c1
DNA Barcode:
GTAGATCAAGCTATAACTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AP4E1 (23431)
Gene Description:
adaptor related protein complex 4 subunit epsilon 1
Transcript:
RefSeq NM_007347.3 (NON-CURRENT)
Match location:
Position 2251 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000382113)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTAGATCAAGCTATAACTAAA
Hairpin Sequence:
5'-CCGG-GTAGATCAAGCTATAACTAAA-CTCGAG-TTTAGTTATAGCTTGATCTAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTAGATCAAGCTATAACTAAACTCGAGTTTAGTTATAGCTTGATCTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTAGATCAAGCTATAACTAAACTCGAGTTTAGTTATAGCTTGATCTAC-3'

Other clones with same target sequence:

(none)