Construct: shRNA TRCN0000382175

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019632.3-844s21c1
DNA Barcode:
GTGAAGGAGTTTGACTCAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Napb (17957)
Gene Description:
N-ethylmaleimide sensitive fusion protein attachment protein beta
Transcript:
RefSeq NM_019632.3 (CURRENT)
Match location:
Position 844 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000382175)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAAGGAGTTTGACTCAATA
Hairpin Sequence:
5'-CCGG-GTGAAGGAGTTTGACTCAATA-CTCGAG-TATTGAGTCAAACTCCTTCAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAAGGAGTTTGACTCAATACTCGAGTATTGAGTCAAACTCCTTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAAGGAGTTTGACTCAATACTCGAGTATTGAGTCAAACTCCTTCAC-3'

Other clones with same target sequence:

(none)