Construct: shRNA TRCN0000382230

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005713.2-949s21c1
DNA Barcode:
ACACGCTACAGAAGTACTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CERT1 (10087)
Gene Description:
ceramide transporter 1
Transcript:
RefSeq NM_005713.2 (NON-CURRENT)
Match location:
Position 949 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000382230)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACACGCTACAGAAGTACTTTG
Hairpin Sequence:
5'-CCGG-ACACGCTACAGAAGTACTTTG-CTCGAG-CAAAGTACTTCTGTAGCGTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACGCTACAGAAGTACTTTGCTCGAGCAAAGTACTTCTGTAGCGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACGCTACAGAAGTACTTTGCTCGAGCAAAGTACTTCTGTAGCGTGT-3'

Other clones with same target sequence:

(none)