Construct: shRNA TRCN0000382297

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000944.4-1864s21c1
DNA Barcode:
AGGAACAAGATCCGAGCAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PPP3CA (5530)
Gene Description:
protein phosphatase 3 catalytic subunit alpha
Transcript:
RefSeq NM_000944.4 (NON-CURRENT)
Match location:
Position 1864 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000382297)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAACAAGATCCGAGCAATA
Hairpin Sequence:
5'-CCGG-AGGAACAAGATCCGAGCAATA-CTCGAG-TATTGCTCGGATCTTGTTCCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAACAAGATCCGAGCAATACTCGAGTATTGCTCGGATCTTGTTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAACAAGATCCGAGCAATACTCGAGTATTGCTCGGATCTTGTTCCT-3'

Other clones with same target sequence:

(none)