Construct: shRNA TRCN0000382337

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001343.2-868s21c1
DNA Barcode:
GAGCATGAACATCCAGTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DAB2 (1601)
Gene Description:
DAB adaptor protein 2
Transcript:
RefSeq NM_001343.2 (NON-CURRENT)
Match location:
Position 868 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000382337)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCATGAACATCCAGTAAAT
Hairpin Sequence:
5'-CCGG-GAGCATGAACATCCAGTAAAT-CTCGAG-ATTTACTGGATGTTCATGCTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCATGAACATCCAGTAAATCTCGAGATTTACTGGATGTTCATGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCATGAACATCCAGTAAATCTCGAGATTTACTGGATGTTCATGCTC-3'

Other clones with same target sequence:

(none)