Construct: shRNA TRCN0000382402

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000195.2-492s21c1
DNA Barcode:
CCTTCACCTGTTTGGAGAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HPS1 (3257)
Gene Description:
HPS1 biogenesis of lysosomal organelles complex 3 subunit 1
Transcript:
RefSeq NM_000195.2 (NON-CURRENT)
Match location:
Position 492 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000382402)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCACCTGTTTGGAGAATG
Hairpin Sequence:
5'-CCGG-CCTTCACCTGTTTGGAGAATG-CTCGAG-CATTCTCCAAACAGGTGAAGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCACCTGTTTGGAGAATGCTCGAGCATTCTCCAAACAGGTGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCACCTGTTTGGAGAATGCTCGAGCATTCTCCAAACAGGTGAAGG-3'

Other clones with same target sequence:

(none)