Construct: shRNA TRCN0000382440

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004333.4-2477s21c1
DNA Barcode:
AGAACACTTGTGTGGTTAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRAF (673)
Gene Description:
B-Raf proto-oncogene, serine/threonine kinase
Transcript:
RefSeq NM_004333.4 (NON-CURRENT)
Match location:
Position 2477 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000382440)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAACACTTGTGTGGTTAAAG
Hairpin Sequence:
5'-CCGG-AGAACACTTGTGTGGTTAAAG-CTCGAG-CTTTAACCACACAAGTGTTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAACACTTGTGTGGTTAAAGCTCGAGCTTTAACCACACAAGTGTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAACACTTGTGTGGTTAAAGCTCGAGCTTTAACCACACAAGTGTTCT-3'

Other clones with same target sequence:

(none)