Construct: shRNA TRCN0000382457

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025134.4-4805s21c1
DNA Barcode:
CCGAGCTCTCTTAGCATATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHD9 (80205)
Gene Description:
chromodomain helicase DNA binding protein 9
Transcript:
RefSeq NM_025134.4 (NON-CURRENT)
Match location:
Position 4805 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000382457)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGCTCTCTTAGCATATTG
Hairpin Sequence:
5'-CCGG-CCGAGCTCTCTTAGCATATTG-CTCGAG-CAATATGCTAAGAGAGCTCGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGCTCTCTTAGCATATTGCTCGAGCAATATGCTAAGAGAGCTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGCTCTCTTAGCATATTGCTCGAGCAATATGCTAAGAGAGCTCGG-3'

Other clones with same target sequence:

(none)