Construct: shRNA TRCN0000382489

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016524.2-709s21c1
DNA Barcode:
CATCGAGTTTGGCGTTCTCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SYT17 (51760)
Gene Description:
synaptotagmin 17
Transcript:
RefSeq NM_016524.2 (NON-CURRENT)
Match location:
Position 709 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000382489)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCGAGTTTGGCGTTCTCAG
Hairpin Sequence:
5'-CCGG-CATCGAGTTTGGCGTTCTCAG-CTCGAG-CTGAGAACGCCAAACTCGATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCGAGTTTGGCGTTCTCAGCTCGAGCTGAGAACGCCAAACTCGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCGAGTTTGGCGTTCTCAGCTCGAGCTGAGAACGCCAAACTCGATG-3'

Other clones with same target sequence:

(none)