Construct: shRNA TRCN0000412386

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145413.4-731s21c1
DNA Barcode:
TCCTAACTGTAGGGAACAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fam20b (215015)
Gene Description:
family with sequence similarity 20, member B
Transcript:
RefSeq NM_145413.4 (CURRENT)
Match location:
Position 731 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412386)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCTAACTGTAGGGAACAATA
Hairpin Sequence:
5'-CCGG-TCCTAACTGTAGGGAACAATA-CTCGAG-TATTGTTCCCTACAGTTAGGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCTAACTGTAGGGAACAATACTCGAGTATTGTTCCCTACAGTTAGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCTAACTGTAGGGAACAATACTCGAGTATTGTTCCCTACAGTTAGGA-3'

Other clones with same target sequence:

(none)