Construct: shRNA TRCN0000412408

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002214.2-1171s21c1
DNA Barcode:
TCAGCATCAATGCACAATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ITGB8 (3696)
Gene Description:
integrin subunit beta 8
Transcript:
RefSeq NM_002214.2 (NON-CURRENT)
Match location:
Position 1171 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412408)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAGCATCAATGCACAATAAT
Hairpin Sequence:
5'-CCGG-TCAGCATCAATGCACAATAAT-CTCGAG-ATTATTGTGCATTGATGCTGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAGCATCAATGCACAATAATCTCGAGATTATTGTGCATTGATGCTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAGCATCAATGCACAATAATCTCGAGATTATTGTGCATTGATGCTGA-3'

Other clones with same target sequence:

(none)