Construct: shRNA TRCN0000412436

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014237.1-399s21c1
DNA Barcode:
AGCAAGATTTGAGCATATAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ADAM18 (8749)
Gene Description:
ADAM metallopeptidase domain 18
Transcript:
RefSeq NM_014237.1 (NON-CURRENT)
Match location:
Position 399 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412436)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAAGATTTGAGCATATAAT
Hairpin Sequence:
5'-CCGG-AGCAAGATTTGAGCATATAAT-CTCGAG-ATTATATGCTCAAATCTTGCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAAGATTTGAGCATATAATCTCGAGATTATATGCTCAAATCTTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAAGATTTGAGCATATAATCTCGAGATTATATGCTCAAATCTTGCT-3'

Other clones with same target sequence:

(none)