Construct: shRNA TRCN0000412510

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145942.4-904s21c1
DNA Barcode:
GATCTTTCACTCACCATATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hmgcs1 (208715)
Gene Description:
3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1
Transcript:
RefSeq NM_145942.4 (NON-CURRENT)
Match location:
Position 904 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412510)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCTTTCACTCACCATATTG
Hairpin Sequence:
5'-CCGG-GATCTTTCACTCACCATATTG-CTCGAG-CAATATGGTGAGTGAAAGATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCTTTCACTCACCATATTGCTCGAGCAATATGGTGAGTGAAAGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCTTTCACTCACCATATTGCTCGAGCAATATGGTGAGTGAAAGATC-3'

Other clones with same target sequence:

  1. TRCN0000218729