Construct: shRNA TRCN0000412621

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_016953.3-1612s21c1
DNA Barcode:
GAATCACCAGTGGTGAAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDE11A (50940)
Gene Description:
phosphodiesterase 11A
Transcript:
RefSeq NM_016953.3 (NON-CURRENT)
Match location:
Position 1612 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412621)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATCACCAGTGGTGAAATTT
Hairpin Sequence:
5'-CCGG-GAATCACCAGTGGTGAAATTT-CTCGAG-AAATTTCACCACTGGTGATTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATCACCAGTGGTGAAATTTCTCGAGAAATTTCACCACTGGTGATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATCACCAGTGGTGAAATTTCTCGAGAAATTTCACCACTGGTGATTC-3'

Other clones with same target sequence:

(none)