Construct: shRNA TRCN0000412632

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000826.3-1184s21c1
DNA Barcode:
TTACTGATGGAGACCTATTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GRIA2 (2891)
Gene Description:
glutamate ionotropic receptor AMPA type subunit 2
Transcript:
RefSeq NM_000826.3 (NON-CURRENT)
Match location:
Position 1184 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412632)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTACTGATGGAGACCTATTAA
Hairpin Sequence:
5'-CCGG-TTACTGATGGAGACCTATTAA-CTCGAG-TTAATAGGTCTCCATCAGTAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTACTGATGGAGACCTATTAACTCGAGTTAATAGGTCTCCATCAGTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTACTGATGGAGACCTATTAACTCGAGTTAATAGGTCTCCATCAGTAA-3'

Other clones with same target sequence:

(none)