Construct: shRNA TRCN0000412639

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003340.5-894s21c1
DNA Barcode:
ATATCTCGGGAATGGACTCAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UBE2D3 (7323)
Gene Description:
ubiquitin conjugating enzyme E2 D3
Transcript:
RefSeq NM_003340.5 (NON-CURRENT)
Match location:
Position 894 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412639)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATATCTCGGGAATGGACTCAG
Hairpin Sequence:
5'-CCGG-ATATCTCGGGAATGGACTCAG-CTCGAG-CTGAGTCCATTCCCGAGATAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATATCTCGGGAATGGACTCAGCTCGAGCTGAGTCCATTCCCGAGATATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATATCTCGGGAATGGACTCAGCTCGAGCTGAGTCCATTCCCGAGATAT-3'

Other clones with same target sequence:

(none)