Construct: shRNA TRCN0000412671
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_198141.2-3251s21c1
- DNA Barcode:
- ACTAACAATATTCCTTGTATC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GANC (2595)
- Gene Description:
- glucosidase alpha, neutral C
- Transcript:
- RefSeq NM_198141.2 (CURRENT)
- Match location:
- Position 3251 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000412671)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- ACTAACAATATTCCTTGTATC
- Hairpin Sequence:
-
5'-CCGG-ACTAACAATATTCCTTGTATC-CTCGAG-GATACAAGGAATATTGTTAGT-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGACTAACAATATTCCTTGTATCCTCGAGGATACAAGGAATATTGTTAGTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAACTAACAATATTCCTTGTATCCTCGAGGATACAAGGAATATTGTTAGT-3'
Other clones with same target sequence:
(none)
