Construct: shRNA TRCN0000412724

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024570.2-528s21c1
DNA Barcode:
ACACCATTCTTGGTTTATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNASEH2B (79621)
Gene Description:
ribonuclease H2 subunit B
Transcript:
RefSeq NM_024570.2 (NON-CURRENT)
Match location:
Position 528 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412724)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACACCATTCTTGGTTTATAAA
Hairpin Sequence:
5'-CCGG-ACACCATTCTTGGTTTATAAA-CTCGAG-TTTATAAACCAAGAATGGTGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACACCATTCTTGGTTTATAAACTCGAGTTTATAAACCAAGAATGGTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACACCATTCTTGGTTTATAAACTCGAGTTTATAAACCAAGAATGGTGT-3'

Other clones with same target sequence:

(none)