Construct: shRNA TRCN0000412774

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178450.3-1137s21c1
DNA Barcode:
TCTGTCAATGTACCTTCTAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MARCHF3 (115123)
Gene Description:
membrane associated ring-CH-type finger 3
Transcript:
RefSeq NM_178450.3 (NON-CURRENT)
Match location:
Position 1137 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412774)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGTCAATGTACCTTCTAAC
Hairpin Sequence:
5'-CCGG-TCTGTCAATGTACCTTCTAAC-CTCGAG-GTTAGAAGGTACATTGACAGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGTCAATGTACCTTCTAACCTCGAGGTTAGAAGGTACATTGACAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGTCAATGTACCTTCTAACCTCGAGGTTAGAAGGTACATTGACAGA-3'

Other clones with same target sequence:

(none)