Construct: shRNA TRCN0000412782

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008091.3-767s21c1
DNA Barcode:
TCCCGTCCTACTACGGAAACT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gata3 (14462)
Gene Description:
GATA binding protein 3
Transcript:
RefSeq NM_008091.3 (CURRENT)
Match location:
Position 767 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412782)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCCGTCCTACTACGGAAACT
Hairpin Sequence:
5'-CCGG-TCCCGTCCTACTACGGAAACT-CTCGAG-AGTTTCCGTAGTAGGACGGGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCCGTCCTACTACGGAAACTCTCGAGAGTTTCCGTAGTAGGACGGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCCGTCCTACTACGGAAACTCTCGAGAGTTTCCGTAGTAGGACGGGA-3'

Other clones with same target sequence:

(none)