Construct: shRNA TRCN0000412801

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_199022.2-2985s21c1
DNA Barcode:
TACGTGCCTTTACCTTGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Shc4 (271849)
Gene Description:
SHC (Src homology 2 domain containing) family, member 4
Transcript:
RefSeq NM_199022.2 (CURRENT)
Match location:
Position 2985 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412801)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACGTGCCTTTACCTTGATAA
Hairpin Sequence:
5'-CCGG-TACGTGCCTTTACCTTGATAA-CTCGAG-TTATCAAGGTAAAGGCACGTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACGTGCCTTTACCTTGATAACTCGAGTTATCAAGGTAAAGGCACGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACGTGCCTTTACCTTGATAACTCGAGTTATCAAGGTAAAGGCACGTA-3'

Other clones with same target sequence:

(none)