Construct: shRNA TRCN0000412803

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013522.3-632s21c1
DNA Barcode:
TGTAATGAAGCAGGCGATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Frg1 (14300)
Gene Description:
FSHD region gene 1
Transcript:
RefSeq NM_013522.3 (CURRENT)
Match location:
Position 632 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412803)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTAATGAAGCAGGCGATATA
Hairpin Sequence:
5'-CCGG-TGTAATGAAGCAGGCGATATA-CTCGAG-TATATCGCCTGCTTCATTACA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTAATGAAGCAGGCGATATACTCGAGTATATCGCCTGCTTCATTACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTAATGAAGCAGGCGATATACTCGAGTATATCGCCTGCTTCATTACA-3'

Other clones with same target sequence:

(none)