Construct: shRNA TRCN0000412806

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014590.3-2141s21c1
DNA Barcode:
CACCTTGCAAGATCAACTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ERVW-1 (30816)
Gene Description:
endogenous retrovirus group W member 1, envelope
Transcript:
RefSeq NM_014590.3 (CURRENT)
Match location:
Position 2141 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412806)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCTTGCAAGATCAACTTAA
Hairpin Sequence:
5'-CCGG-CACCTTGCAAGATCAACTTAA-CTCGAG-TTAAGTTGATCTTGCAAGGTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCTTGCAAGATCAACTTAACTCGAGTTAAGTTGATCTTGCAAGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCTTGCAAGATCAACTTAACTCGAGTTAAGTTGATCTTGCAAGGTG-3'

Other clones with same target sequence:

(none)