Construct: shRNA TRCN0000412823

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080657.4-374s21c1
DNA Barcode:
ACTCGCCAGTGCAACTACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RSAD2 (91543)
Gene Description:
radical S-adenosyl methionine domain containing 2
Transcript:
RefSeq NM_080657.4 (NON-CURRENT)
Match location:
Position 374 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412823)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTCGCCAGTGCAACTACAAA
Hairpin Sequence:
5'-CCGG-ACTCGCCAGTGCAACTACAAA-CTCGAG-TTTGTAGTTGCACTGGCGAGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTCGCCAGTGCAACTACAAACTCGAGTTTGTAGTTGCACTGGCGAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTCGCCAGTGCAACTACAAACTCGAGTTTGTAGTTGCACTGGCGAGT-3'

Other clones with same target sequence:

(none)