Construct: shRNA TRCN0000412860

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133207.2-1572s21c1
DNA Barcode:
AGACTTGATTGTGGATATTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kcnh7 (170738)
Gene Description:
potassium voltage-gated channel, subfamily H (eag-related), member 7
Transcript:
RefSeq NM_133207.2 (CURRENT)
Match location:
Position 1572 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412860)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACTTGATTGTGGATATTAT
Hairpin Sequence:
5'-CCGG-AGACTTGATTGTGGATATTAT-CTCGAG-ATAATATCCACAATCAAGTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACTTGATTGTGGATATTATCTCGAGATAATATCCACAATCAAGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACTTGATTGTGGATATTATCTCGAGATAATATCCACAATCAAGTCT-3'

Other clones with same target sequence:

(none)