Construct: shRNA TRCN0000412881

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_174937.3-2153s21c1
DNA Barcode:
ACGGGAGCCTCTGTAACAATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TCERG1L (256536)
Gene Description:
transcription elongation regulator 1 like
Transcript:
RefSeq NM_174937.3 (NON-CURRENT)
Match location:
Position 2153 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412881)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGGGAGCCTCTGTAACAATC
Hairpin Sequence:
5'-CCGG-ACGGGAGCCTCTGTAACAATC-CTCGAG-GATTGTTACAGAGGCTCCCGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGGGAGCCTCTGTAACAATCCTCGAGGATTGTTACAGAGGCTCCCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGGGAGCCTCTGTAACAATCCTCGAGGATTGTTACAGAGGCTCCCGT-3'

Other clones with same target sequence:

(none)