Construct: shRNA TRCN0000412894

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000555.2-1685s21c1
DNA Barcode:
GATCCACAGTTACCAATTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DCX (1641)
Gene Description:
doublecortin
Transcript:
RefSeq NM_000555.2 (NON-CURRENT)
Match location:
Position 1685 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412894)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCCACAGTTACCAATTATG
Hairpin Sequence:
5'-CCGG-GATCCACAGTTACCAATTATG-CTCGAG-CATAATTGGTAACTGTGGATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCCACAGTTACCAATTATGCTCGAGCATAATTGGTAACTGTGGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCCACAGTTACCAATTATGCTCGAGCATAATTGGTAACTGTGGATC-3'

Other clones with same target sequence:

(none)