Construct: shRNA TRCN0000412926

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018409.3-1765s21c1
DNA Barcode:
CAACCGCTAAACACTATTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRP2BP (55805)
Gene Description:
LRP2 binding protein
Transcript:
RefSeq NM_018409.3 (CURRENT)
Match location:
Position 1765 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412926)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACCGCTAAACACTATTATT
Hairpin Sequence:
5'-CCGG-CAACCGCTAAACACTATTATT-CTCGAG-AATAATAGTGTTTAGCGGTTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACCGCTAAACACTATTATTCTCGAGAATAATAGTGTTTAGCGGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACCGCTAAACACTATTATTCTCGAGAATAATAGTGTTTAGCGGTTG-3'

Other clones with same target sequence:

(none)