Construct: shRNA TRCN0000412980

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000102.3-759s21c1
DNA Barcode:
ATGTCATACAGAATTACAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYP17A1 (1586)
Gene Description:
cytochrome P450 family 17 subfamily A member 1
Transcript:
RefSeq NM_000102.3 (NON-CURRENT)
Match location:
Position 759 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412980)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTCATACAGAATTACAATG
Hairpin Sequence:
5'-CCGG-ATGTCATACAGAATTACAATG-CTCGAG-CATTGTAATTCTGTATGACAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTCATACAGAATTACAATGCTCGAGCATTGTAATTCTGTATGACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTCATACAGAATTACAATGCTCGAGCATTGTAATTCTGTATGACAT-3'

Other clones with same target sequence:

(none)