Construct: shRNA TRCN0000412999

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032622.2-1524s21c1
DNA Barcode:
GTCATGAGAAGGTGGTAAATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LNX1 (84708)
Gene Description:
ligand of numb-protein X 1
Transcript:
RefSeq NM_032622.2 (CURRENT)
Match location:
Position 1524 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000412999)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCATGAGAAGGTGGTAAATA
Hairpin Sequence:
5'-CCGG-GTCATGAGAAGGTGGTAAATA-CTCGAG-TATTTACCACCTTCTCATGAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCATGAGAAGGTGGTAAATACTCGAGTATTTACCACCTTCTCATGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCATGAGAAGGTGGTAAATACTCGAGTATTTACCACCTTCTCATGAC-3'

Other clones with same target sequence:

(none)