Construct: shRNA TRCN0000413015

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173653.2-1399s21c1
DNA Barcode:
TTGCCAGAGCCTGCAACATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC9A9 (285195)
Gene Description:
solute carrier family 9 member A9
Transcript:
RefSeq NM_173653.2 (NON-CURRENT)
Match location:
Position 1399 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413015)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGCCAGAGCCTGCAACATAT
Hairpin Sequence:
5'-CCGG-TTGCCAGAGCCTGCAACATAT-CTCGAG-ATATGTTGCAGGCTCTGGCAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGCCAGAGCCTGCAACATATCTCGAGATATGTTGCAGGCTCTGGCAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGCCAGAGCCTGCAACATATCTCGAGATATGTTGCAGGCTCTGGCAA-3'

Other clones with same target sequence:

(none)