Construct: shRNA TRCN0000413045

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022132.4-2056s21c1
DNA Barcode:
TAAAGCGGAGACAGTAATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MCCC2 (64087)
Gene Description:
methylcrotonoyl-CoA carboxylase 2
Transcript:
RefSeq NM_022132.4 (NON-CURRENT)
Match location:
Position 2056 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413045)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAAGCGGAGACAGTAATTTA
Hairpin Sequence:
5'-CCGG-TAAAGCGGAGACAGTAATTTA-CTCGAG-TAAATTACTGTCTCCGCTTTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAAGCGGAGACAGTAATTTACTCGAGTAAATTACTGTCTCCGCTTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAAGCGGAGACAGTAATTTACTCGAGTAAATTACTGTCTCCGCTTTA-3'

Other clones with same target sequence:

(none)