Construct: shRNA TRCN0000413080

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024080.4-3535s21c1
DNA Barcode:
TCATACTTGAAGACGGATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRPM8 (79054)
Gene Description:
transient receptor potential cation channel subfamily M member 8
Transcript:
RefSeq NM_024080.4 (NON-CURRENT)
Match location:
Position 3535 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413080)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCATACTTGAAGACGGATATA
Hairpin Sequence:
5'-CCGG-TCATACTTGAAGACGGATATA-CTCGAG-TATATCCGTCTTCAAGTATGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCATACTTGAAGACGGATATACTCGAGTATATCCGTCTTCAAGTATGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCATACTTGAAGACGGATATACTCGAGTATATCCGTCTTCAAGTATGA-3'

Other clones with same target sequence:

(none)