Construct: shRNA TRCN0000413130

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001715.2-2344s21c1
DNA Barcode:
ACGGTCATCCGGAGTACTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BLK (640)
Gene Description:
BLK proto-oncogene, Src family tyrosine kinase
Transcript:
RefSeq NM_001715.2 (NON-CURRENT)
Match location:
Position 2344 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413130)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGGTCATCCGGAGTACTAAG
Hairpin Sequence:
5'-CCGG-ACGGTCATCCGGAGTACTAAG-CTCGAG-CTTAGTACTCCGGATGACCGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGGTCATCCGGAGTACTAAGCTCGAGCTTAGTACTCCGGATGACCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGGTCATCCGGAGTACTAAGCTCGAGCTTAGTACTCCGGATGACCGT-3'

Other clones with same target sequence:

(none)