Construct: shRNA TRCN0000413212

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018190.2-1371s21c1
DNA Barcode:
GGTACAGACTGCAATAGATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BBS7 (55212)
Gene Description:
Bardet-Biedl syndrome 7
Transcript:
RefSeq NM_018190.2 (NON-CURRENT)
Match location:
Position 1371 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413212)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTACAGACTGCAATAGATAA
Hairpin Sequence:
5'-CCGG-GGTACAGACTGCAATAGATAA-CTCGAG-TTATCTATTGCAGTCTGTACC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTACAGACTGCAATAGATAACTCGAGTTATCTATTGCAGTCTGTACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTACAGACTGCAATAGATAACTCGAGTTATCTATTGCAGTCTGTACC-3'

Other clones with same target sequence:

(none)