Construct: shRNA TRCN0000413253

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003786.3-3809s21c1
DNA Barcode:
GATACGAATGATGTCAGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABCC3 (8714)
Gene Description:
ATP binding cassette subfamily C member 3
Transcript:
RefSeq NM_003786.3 (NON-CURRENT)
Match location:
Position 3809 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413253)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATACGAATGATGTCAGATTT
Hairpin Sequence:
5'-CCGG-GATACGAATGATGTCAGATTT-CTCGAG-AAATCTGACATCATTCGTATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATACGAATGATGTCAGATTTCTCGAGAAATCTGACATCATTCGTATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATACGAATGATGTCAGATTTCTCGAGAAATCTGACATCATTCGTATC-3'

Other clones with same target sequence:

(none)