Construct: shRNA TRCN0000413368

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006362.4-2162s21c1
DNA Barcode:
TCAGAAGATTGAGCCTCACTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NXF1 (10482)
Gene Description:
nuclear RNA export factor 1
Transcript:
RefSeq NM_006362.4 (NON-CURRENT)
Match location:
Position 2162 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413368)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAGAAGATTGAGCCTCACTG
Hairpin Sequence:
5'-CCGG-TCAGAAGATTGAGCCTCACTG-CTCGAG-CAGTGAGGCTCAATCTTCTGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAGAAGATTGAGCCTCACTGCTCGAGCAGTGAGGCTCAATCTTCTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAGAAGATTGAGCCTCACTGCTCGAGCAGTGAGGCTCAATCTTCTGA-3'

Other clones with same target sequence:

(none)