Construct: shRNA TRCN0000413382

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011131.3-1615s21c1
DNA Barcode:
CCGACTACTAGAGCGCCTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pold1 (18971)
Gene Description:
polymerase (DNA directed), delta 1, catalytic subunit
Transcript:
RefSeq NM_011131.3 (CURRENT)
Match location:
Position 1615 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413382)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGACTACTAGAGCGCCTTAT
Hairpin Sequence:
5'-CCGG-CCGACTACTAGAGCGCCTTAT-CTCGAG-ATAAGGCGCTCTAGTAGTCGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGACTACTAGAGCGCCTTATCTCGAGATAAGGCGCTCTAGTAGTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGACTACTAGAGCGCCTTATCTCGAGATAAGGCGCTCTAGTAGTCGG-3'

Other clones with same target sequence:

(none)