Construct: shRNA TRCN0000413383

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008262.3-933s21c1
DNA Barcode:
GTCTGGGCAGCATTCACAACT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Onecut1 (15379)
Gene Description:
one cut domain, family member 1
Transcript:
RefSeq NM_008262.3 (CURRENT)
Match location:
Position 933 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413383)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTGGGCAGCATTCACAACT
Hairpin Sequence:
5'-CCGG-GTCTGGGCAGCATTCACAACT-CTCGAG-AGTTGTGAATGCTGCCCAGAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTGGGCAGCATTCACAACTCTCGAGAGTTGTGAATGCTGCCCAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTGGGCAGCATTCACAACTCTCGAGAGTTGTGAATGCTGCCCAGAC-3'

Other clones with same target sequence:

(none)