Construct: shRNA TRCN0000413468

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018261.3-2097s21c1
DNA Barcode:
TTTGACAAATGCATTAGTAAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EXOC1 (55763)
Gene Description:
exocyst complex component 1
Transcript:
RefSeq NM_018261.3 (CURRENT)
Match location:
Position 2097 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413468)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTGACAAATGCATTAGTAAC
Hairpin Sequence:
5'-CCGG-TTTGACAAATGCATTAGTAAC-CTCGAG-GTTACTAATGCATTTGTCAAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTGACAAATGCATTAGTAACCTCGAGGTTACTAATGCATTTGTCAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTGACAAATGCATTAGTAACCTCGAGGTTACTAATGCATTTGTCAAA-3'

Other clones with same target sequence:

(none)