Construct: shRNA TRCN0000413549

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001002294.2-1903s21c1
DNA Barcode:
TTAAGAGAGCACTAATCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FMO3 (2328)
Gene Description:
flavin containing dimethylaniline monoxygenase 3
Transcript:
RefSeq NM_001002294.2 (NON-CURRENT)
Match location:
Position 1903 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413549)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTAAGAGAGCACTAATCATTT
Hairpin Sequence:
5'-CCGG-TTAAGAGAGCACTAATCATTT-CTCGAG-AAATGATTAGTGCTCTCTTAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTAAGAGAGCACTAATCATTTCTCGAGAAATGATTAGTGCTCTCTTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTAAGAGAGCACTAATCATTTCTCGAGAAATGATTAGTGCTCTCTTAA-3'

Other clones with same target sequence:

(none)