Construct: shRNA TRCN0000413576

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_013347.3-1122s21c1
DNA Barcode:
GCATCCAGCTGTGAGTAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPA4 (29935)
Gene Description:
replication protein A4
Transcript:
RefSeq NM_013347.3 (NON-CURRENT)
Match location:
Position 1122 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000413576)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATCCAGCTGTGAGTAATTT
Hairpin Sequence:
5'-CCGG-GCATCCAGCTGTGAGTAATTT-CTCGAG-AAATTACTCACAGCTGGATGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATCCAGCTGTGAGTAATTTCTCGAGAAATTACTCACAGCTGGATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATCCAGCTGTGAGTAATTTCTCGAGAAATTACTCACAGCTGGATGC-3'

Other clones with same target sequence:

(none)