Construct: shRNA TRCN0000413576
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_013347.3-1122s21c1
- DNA Barcode:
- GCATCCAGCTGTGAGTAATTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- RPA4 (29935)
- Gene Description:
- replication protein A4
- Transcript:
- RefSeq NM_013347.3 (NON-CURRENT)
- Match location:
- Position 1122 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000413576)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCATCCAGCTGTGAGTAATTT
- Hairpin Sequence:
-
5'-CCGG-GCATCCAGCTGTGAGTAATTT-CTCGAG-AAATTACTCACAGCTGGATGC-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCATCCAGCTGTGAGTAATTTCTCGAGAAATTACTCACAGCTGGATGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCATCCAGCTGTGAGTAATTTCTCGAGAAATTACTCACAGCTGGATGC-3'
Other clones with same target sequence:
(none)
